Australian Drosophila Transgenic Facility (ADTF)


The Australian Drosophila Transgenic Facility (ADTF), supported by Phenomics Australia and Peter MacCallum Cancer Centre, offers specialised microinjection services for generating transgenic strains of the fruit fly Drosophila melanogaster.

 

Service Overview

This facility currently offers the following approaches for transgenesis in D. melanogaster:

  • PhiC31 integrase-mediated transgenesis ($500): A highly efficient approach enabling site-specific integration of transgenes into a desired location (attP40 & attP2 landing sites) within the D. melanogaster genome. Available stocks include the attP2 (w nanos-int; P{CaryP}attP2) and the attP40 (y w vas-int; P{CaryP}attP40) (Note: vasa promoter marked with GFP and RFP in the eye).
  • P-element-mediated transgenesis ($600): A classical method involving the introduction of transgenes via the P-element transposon system. It is important to note that this method leads to random integration, potentially resulting in positional effects that can influence the activity of transgenes.

     

Getting Your Project Underway

Starting your request:

  • If you're a first-time user, create an iLab account to get started. If you need any assistance with registration process please consult our guide or contact us.
  • Use the "Request Services" tab to submit your service request.
  • Once your request is approved, please send us your purified plasmid samples (with a concentration of at least 300 ng/µl and a total volume of at least 30 µl) to the following address:

 

Peter MacCallum Cancer Centre
Alex Giang
305 Grattan Street
Melbourne VIC 3000

 

The workflow:

  • After receiving your plasmid sample, we'll commence work on your order.
  • Our service covers microinjection, followed by screening, and then dispatching the transgenic flies to you.
  • We'll provide milestone updates throughout the process.

 

Please do not hestiate to contact us if you have any questions!

alex.giang@petermac.org

astrid.nissila-browne@petermac.org

 

Our Team

Co-head: Professor Kieran Harvey and Associate Professor Louise Cheng

Facility Operator / Technician: Dr Alex Giang and Astrid Nissila-Browne

 

Links and Resources

 

Acknowledgments

The ADTF is based at the Peter MacCallum Cancer Centre and is supported by the Federal Government through the National Collaborative Research Infrastructure Strategy (NCRIS) initiative and by Phenomics Australia.

PMAC MASTER RGB HORIZONTAL    PhenomicsAustralia PrecisionMedicine LOGOs        NCRIS PROVIDER

Contacts

Name Role Phone Email Location
Alex Giang
Facility Operator/Technician
 

 
alex.giang@petermac.org
 
305 Grattan Street, Melbourne VIC 3000
 
Astrid Nissila-Browne
Facility Operator/Technician
 

 
astrid.nissila-browne@petermac.org
 
305 Grattan Street, Melbourne VIC 3000