Australian Drosophila Transgenic Facility (ADTF)

The Australian Drosophila Transgenic Facility (ADTF), supported by Phenomics Australia and Peter MacCallum Cancer Centre, offers specialised microinjection services for generating transgenic strains of the fruit fly Drosophila melanogaster.

Service Overview

This facility currently offers the following approaches for transgenesis in D. melanogaster:

• PhiC31 integrase-mediated transgenesis ($500): A highly efficient approach enabling site-specific integration of transgenes into the D. melanogaster genome at predefined locations. Available stocks include:
  • attP2 (y w nanos-int; P{CaryP}attP2).
  • attP40 (y w vas-int; P{CaryP}attP40) (Note: vas-int is marked with GFP and RFP in the eyes).
  • We also maintain vermillion stocks for flies with these attP integration sites (BDSC#25709 and BDSC#25710).

• P-element-mediated transgenesis ($600): A classical method involving the introduction of transgenes via the P-element transposon system. It is important to note that this method leads to random integration, potentially resulting in positional effects that can influence the activity of transgenes.

   

Getting Your Project Underway

Starting your request:

• If you're a first-time user, create an iLab account to get started. If you need any assistance with registration process please consult our guide or contact us.
• Use the "Request Services" tab to submit your service request.
• Once your request is approved, please send us your purified plasmid samples in pure water (with a concentration of at least 400 ng/µl and a total volume of at least 30 µl) to the following address:

The workflow:

• After receiving your plasmid sample, we'll commence work on your order.
• Our service covers microinjection, followed by screening, and then dispatching the transgenic flies to you.
• We'll provide milestone updates throughout the process.

Please do not hestiate to contact us if you have any questions!

alex.giang@petermac.org

astrid.nissila-browne@petermac.org

Our Team

Co-head: Professor Kieran Harvey and Associate Professor Louise Cheng

Facility Operator / Technician: Dr Alex Giang and Astrid Nissila-Browne

Links and Resources

• ADTF Site
• ADTF iLAB Registration Guide
• ADTF Information Booklet

Acknowledgments

The ADTF is based at the Peter MacCallum Cancer Centre and is supported by the Federal Government through the National Collaborative Research Infrastructure Strategy (NCRIS) initiative and by Phenomics Australia.

Contacts