For licensed users who would like assistance in setting up their first experiment post licensing or when starting a new project. 

This will include assistance with setting up your worksheet (plots and gates), baseline voltages and compensation, this is a 30min booking if you think you need more please let us know in your booking. 

For licensed users who would like a training session on how to operate and set up the HTS which is on selected analysers:

• Verse
• LSR II
• Fortessa
• Symphony A3

All users must be fully licensed through this course and practical training before they are permitted to use any of the analysis instruments. 

PeterMac Research FlowCore (RFC) provides comprehensive training in: 

• The basic theory of flow cytometry; including fluidics, optics, and how data is generated.
• Instrument operation; including correct start-up, shutdown, and troubleshooting.
• Sample preparation - good data generation is impossible without careful sample preparation. Staff are more than happy to discuss topics such as cell preparation and staining for you to generate good data, and importantly, maximising your output. 

Practical training will consist of several separate sessions where you will be taught and demonstrate how to correctly use the instruments and the basics of setting up a 4-colour flow experiment. 

Please do not initiate training until you are ready to complete ALL aspects of training, including instrument induction and instrument training.  

The process of commencing training to licensing should be completed within a month.  

No licensing will be carried out in December if training has not been initiated by mid-November. 

Training can be found on our Learning Management System (LMS) https://learninghub.petermac.org.au/course/view.php?id=758 under ‘Research FlowCore Induction Training.’

Please email ResearchFlowCore@petermac.org if you do not have access to LMS and/or you are an external/VCCC partner staff member. 

The Induction training consists of 3 parts:

- Syllabus 
- Quiz
- Video

Once LMS is completed: Speak with your supervisor and decide which instrument will be most suited for your scientific applications (Specs of our instruments may be found here Research Flow Core (corefacilities.org), and contact us at researchflowcore@petermac.org with your Certificate of Completion attached to book a time to start your practical training sessions. Training sessions will continue until you can independently perform a 4-colour flow experiment shown in the video including compensation and will finish with a licensing test where you will perform the experiment without staff assistance.

Once you are licensed you have the opportunity to book an experimental setup session where a staff member will assist with the gating, compensation, and setup to create a robust template for your future experiments.

Training sessions are $80/hr for each 1-1 sessions, if you need a refresher session, please book a Refresher training session.

Eva, Elle, Fatma, and Lara are also happy to provide guidance on polychromatic panel design and other flow topics after your training is completed, please remember that the facility must be acknowledged in any published work through the facility and if any staff member contributes intellectual input into your experiment they should receive authorship.

For users who have previoulsy completed licensing at PeterMac FlowCore and due to inactivity would like a 30min refresher training on an analysis platform. 

This training is for licensed users who have been trained but would like to use a different analysis platform with different fluidics/operations. 

• If you have been trained on the Canto or Verse you will need step up training to use any of the other instuments. 
• If you have been trained on the LSR, Fortessa, A3 or A5 you can use any of these platforms but will need a step up training to use the Canto and Verse

All users must be fully licensed through this course and practical training before they are permitted to use any of the analysis instruments. 

PeterMac Research FlowCore (RFC) provides comprehensive training in: 

• The basic theory of flow cytometry; including fluidics, optics, and how data is generated.
• Instrument operation; including correct start-up, shutdown, and troubleshooting.
• Sample preparation - good data generation is impossible without careful sample preparation. Staff are more than happy to discuss topics such as cell preparation and staining for you to generate good data, and importantly, maximising your output. 

Practical training will consist of several separate sessions where you will be taught and demonstrate how to correctly use the instruments and the basics of setting up a 4-colour flow experiment. 

Please do not initiate training until you are ready to complete ALL aspects of training, including instrument induction and instrument training.  

The process of commencing training to licensing should be completed within a month.  

No licensing will be carried out in December if training has not been initiated by mid-November. 

Training can be found on our Learning Management System (LMS) https://learninghub.petermac.org.au/course/view.php?id=758 under ‘Research FlowCore Induction Training.’

Please email ResearchFlowCore@petermac.org if you do not have access to LMS and/or you are an external/VCCC partner staff member. 

The Induction training consists of 3 parts:

- Syllabus 
- Quiz
- Video

Once LMS is completed: Speak with your supervisor and decide which instrument will be most suited for your scientific applications (Specs of our instruments may be found here Research Flow Core (corefacilities.org), and contact us at researchflowcore@petermac.org with your Certificate of Completion attached to book a time to start your practical training sessions. Training sessions will continue until you can independently perform a 4-colour flow experiment shown in the video including compensation and will finish with a licensing test where you will perform the experiment without staff assistance.

Once you are licensed you have the opportunity to book an experimental setup session where a staff member will assist with the gating, compensation, and setup to create a robust template for your future experiments.

Training sessions are $80/hr for each 1-1 sessions, if you need a refresher session, please book a Refresher training session.

Eva, Elle, Fatma, and Lara are also happy to provide guidance on polychromatic panel design and other flow topics after your training is completed, please remember that the facility must be acknowledged in any published work through the facility and if any staff member contributes intellectual input into your experiment they should receive authorship.

For licensed users who would like a training session on how to operate and set up the HTS which is on selected analysers:

• Verse
• LSR II
• Fortessa
• Symphony A3

For licensed users who would like assistance in setting up their first experiment post licensing or when starting a new project. 

This will include assistance with setting up your worksheet (plots and gates), baseline voltages and compensation, this is a 30min booking if you think you need more please let us know in your booking. 

For users who have previoulsy completed licensing at PeterMac FlowCore and due to inactivity would like a 30min refresher training on an analysis platform. 

This training is for licensed users who have been trained but would like to use a different analysis platform with different fluidics/operations. 

• If you have been trained on the Canto or Verse you will need step up training to use any of the other instuments. 
• If you have been trained on the LSR, Fortessa, A3 or A5 you can use any of these platforms but will need a step up training to use the Canto and Verse